Objectives

Filter Sets

Axio Observer Z1 inverted microscope with motorized stage

34 channel detector

·         2 standard PMTs

·         32 channel GaAsP detector with 45% quantum efficiency

·         Airyscan detector (coming June 2017)

Lasers

Software packages and add-ons

·         Definite Focus

·         Experiment Designer module

·         Tiles/Positions Module

·         Temperature control module

·         Plexiglas microscope housing

·         CO2 control module

·         Capabilities

                 o   Time lapse with Definite Focus

                 o   Z scanning

                 o   Tile scanning

                 o   ROI bleaching

                 o   Linear unmixing

Other assets

·         Online reservation system

·         Computer workstation with full software capability

·         Axio Observer Z1 inverted microscope for fluorescence or brightfield microscopy

·         Biosafety cabinet & CO2 incubator in adjoining lab

 

 

 

Laser

 

 

 

 

Power (mW)

 

 

 

 

Wavelength (nm)

 

 

 

 

Example fluors

 

 

 

 

Near UV

 

 

 

 

35

 

 

 

 

405

 

 

 

 

DAPI, Hoechst, Cascade Blue/Yellow, most UV-excitable dyes

 

 

 

 

Argon

 

 

 

 

25

 

 

 

 

458

 

 

 

 

LuY, BOBO-1, CFP

 

 

 

 

488

 

 

 

 

Cy2, GFP, FITC, Alexa Fluor 488, PE, YOYO-1

 

 

 

 

514

 

 

 

 

YFP

 

 

 

 

HeNe1

 

 

 

 

1

 

 

 

 

543

 

 

 

 

PE, Alexa Fluor 546, rhodamine, Cy3, DsRed, PI

 

 

 

 

HeNe2

 

 

 

 

2

 

 

 

 

594

 

 

 

 

Alexa Fluor 594, Cy3.5, Texas Red

 

 

 

 

HeNe3

 

 

 

 

5

 

 

 

 

633

 

 

 

 

Cy5, Alexa Fluor 633, Alexa Fluor 647