The researcher isolates DNA templates from a variety of sources, including plasmids and PCR reactions and submits samples pre-mixed with primers, based on the requirements specified by GSC. GSC performs sequencing on ABI 3730 DNA Analyzer and deliver the ab1 files electronically.
Sanger Full Reactions
GSC staff set up cycle sequencing reactions, perform clean-up, run the samples on the ABI 3730, and deliver the sequence data electronically.
In order to ensure the best quality results, samples submitted for analysis must meet certain requirements. Detailed guidelines for sample preparation can be found in our downloadable Sample Submission Requirements Guide.
Creating and Managing New Projects (GSC LIMS)
All Sanger sequencing requests must now go through our GSC Laboratory Information Management System (LIMS). If this is your first time using LIMS, please follow the instructions in our LIMS User Guide to create a new account and verify your payment method. You must have a GSC LIMS account and login to access the new submission forms.
If you have any questions about your LIMS account or the sample submission process, please email Kris Lee at email@example.com or call (540) 231-1229.
In person: Customers can do so at the Steger Hall main reception desk between the hours of 8:00 AM and 5:00 PM. In most cases, samples received by 8:30 AM will be processed that same day.
By Shuttle from VTCRI: We have a protocol for transporting DNA and RNA samples (both Sanger and NGS) Download the Fralin Biomedical Research Institute Sample Transfer Protocol by Shuttle.
By Mail: Pack the samples with ice packs and include an information sheet with the researcher’s name and contact information and indicate that the samples are intended for Sanger sequencing. Send the package to:
Genomics Sequencing Center
Fralin Life Sciences Institute at Virginia Tech
1015 Life Science Circle
Blacksburg, VA 24061
Upon completion of the sequencing run, you will be notified via email through the LIMS that your results are available for you to download. Log into your LIMS account and save these files (.ab1 files) to your computer. There are several software programs available online for viewing this type of sequencing file. For Sanger sequencing, we use Sequence Scanner 2 which is free downloadable software available from ABI.
Monday through Friday, our turnaround time is 48 hours, but we make every effort to complete the work within 24 hours. Our lab is closed on weekends so any Sanger samples submitted after 8:30 AM on Friday will not be processed until Monday, with results going out on Tuesday.