Mechanisms of Membrane Curvature Generation

Baumgart

 

Tobias Baumgart; Physical & Biological Chemistry, University of Pennsylvania

September 22 in the Fralin Auditorium, Fralin Hall 102

Hosted by  Dr. Daniel Capelluto

 

 

Membrane curvature has developed into a forefront of membrane biophysics. Numerous proteins involved in membrane curvature sensing and membrane curvature generation have recently been discovered, including proteins containing the crescent-shaped BAR domain as membrane binding and shaping module. Accordingly, the structure of these proteins and their multimeric complexes is increasingly well-understood. Substantially less understood, however, are the detailed mechanisms of how these proteins interact with membranes in a curvature-dependent manner. New experimental approaches need to be combined with established techniques to be able to fill in these missing details. Here we use model membrane systems in combination with a variety of biophysical techniques to characterize mechanistic aspects of BAR domain protein function. This includes a characterization of membrane curvature sensing and membrane generation. We present a new approach to investigate membrane curvature transitions, and introduce membrane shape stability diagrams as a powerful tool to enhance the mechanistic understanding of membrane trafficking phenomena, including endocytosis, with molecular detail.

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Chen JACS 2016.pdf The N‐Terminal Amphipathic Helix of Endophilin Does Not Contribute to Its Molecular Curvature Generation Capacity
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Graber PCCP 2017.pdf Cations induce shape remodeling of negatively charged phospholipid membranes

This seminar will NOT be livestreamed or archived.

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