Mechanisms of Membrane Curvature Generation



Tobias Baumgart; Physical & Biological Chemistry, University of Pennsylvania

September 22 in the Fralin Auditorium, Fralin Hall 102

Hosted by  Dr. Daniel Capelluto



Membrane curvature has developed into a forefront of membrane biophysics. Numerous proteins involved in membrane curvature sensing and membrane curvature generation have recently been discovered, including proteins containing the crescent-shaped BAR domain as membrane binding and shaping module. Accordingly, the structure of these proteins and their multimeric complexes is increasingly well-understood. Substantially less understood, however, are the detailed mechanisms of how these proteins interact with membranes in a curvature-dependent manner. New experimental approaches need to be combined with established techniques to be able to fill in these missing details. Here we use model membrane systems in combination with a variety of biophysical techniques to characterize mechanistic aspects of BAR domain protein function. This includes a characterization of membrane curvature sensing and membrane generation. We present a new approach to investigate membrane curvature transitions, and introduce membrane shape stability diagrams as a powerful tool to enhance the mechanistic understanding of membrane trafficking phenomena, including endocytosis, with molecular detail.

Chen JACS 2016.pdf The N‐Terminal Amphipathic Helix of Endophilin Does Not Contribute to Its Molecular Curvature Generation Capacity
Graber PCCP 2017.pdf Cations induce shape remodeling of negatively charged phospholipid membranes

This seminar will NOT be livestreamed or archived.

Baumgart flyer